ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (7): 1381-1388.doi: 10.11843/j.issn.0366-6964.2016.07.010

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The Effect of miR-324-3p on Melanin Synthesis via MC1R Gene

ZENG Qing-bao,FAN Rui-wen,ZHANG Qiu-yue,HAO Xiao-juan,WU Liang-qi,REN Yu-hong*   

  1. (College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
  • Received:2015-12-30 Online:2016-07-23 Published:2016-07-23

Abstract:

To investigate the effect of miR-324-3p on regulating melanin production by targeting MC1R,miR-324-3p was over-expressed by liposome transfection method in alpaca melanocytes in vitro.The expression of MC1R and hair color genes (Mitf,Tyr and Tyrp2) were detected by quantitative real time PCR and Western blotting and the melanin prodution was analyzed by Microplate Reader.The results showed that miR-324-3p was expressed extremely significant difference in the alpaca skins with 1.64 folds in brown alpaca skins vs white alpaca skins(P<0.01),the gene expression of MC1R,Mitf,Tyr and Tyrp2 were down-regulated and the melanin production was reduced(especially Mitf (P<0.01)).The results suggested that miR-324-3p regulated melanogenesis by targeting MC1R gene to down-regulate the expression of the downstream genes including Mitf,Tyr and Tyrp2 in the skin of alpaca.

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